A Competitive Nucleotide Binding Inhibitor: In Vitro Characterization of Rab7 GTPase Inhibition

Multiple Roles of the Small GTPase Rab7

Rab7 is a small GTPase that belongs to the Rab family and controls transport to late endocytic compartments such as late endosomes and lysosomes. The mechanism of action of Rab7 in the late endocytic pathway has been extensively studied. Rab7 is fundamental for lysosomal biogenesis, positioning and functions, and for trafficking and degradation of several signaling receptors, thus also having i...

Structural basis for recruitment of RILP by small GTPase Rab7.

Rab7 regulates vesicle traffic from early to late endosomes, and from late endosomes to lysosomes. The crystal structure of Rab7-GTP in complex with the Rab7 binding domain of RILP reveals that Rab7 interacts with RILP specifically via two distinct areas, with the first one involving the switch and interswitch regions and the second one consisting of RabSF1 and RabSF4. Disruption of these inter...

Induced nucleotide specificity in a GTPase.

In signal-recognition particle (SRP)-dependent protein targeting to the bacterial plasma membrane, two GTPases, Ffh (a subunit of the bacterial SRP) and FtsY (the bacterial SRP receptor), act as GTPase activating proteins for one another. The molecular mechanism of this reciprocal GTPase activation is poorly understood. In this work, we show that, unlike other GTPases, free FtsY exhibits only l...

Enzyme-Linked Immunosorbent Assay Competitive Binding Inhibition

Corrosion Inhibition of Mild Steel in Acidic Solution by Apricot Gum as a Green Inhibitor

The inhibitive effect of apricot gum AG on mild steel in 0.5 M phosphoric acid solution was investigated by potentiodynamic polarization, electrochemical impedance spectroscopy EIS, linear polarization resistance and electrochemical noise EN techniques. The inhibition efficiency increased with increasing AG concentration up to a certain value and decreased with increasing temperature. Polarizat...